Measurement of intracellular reactive oxygen species in mature single skeletal muscle fibres by dichlorofluorescein‐based fluorescence microscopy
نویسندگان
چکیده
منابع مشابه
Reactive Oxygen Species in Skeletal Muscle Signaling
Generation of reactive oxygen species (ROS) is a ubiquitous phenomenon in eukaryotic cells' life. Up to the 1990s of the past century, ROS have been solely considered as toxic species resulting in oxidative stress, pathogenesis and aging. However, there is now clear evidence that ROS are not merely toxic species but also-within certain concentrations-useful signaling molecules regulating physio...
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The existence of hypoxia-induced reactive oxygen species (ROS) production remains controversial. However, numerous observations with a variety of methods and in many cells and tissue types are supportive of this idea. Skeletal muscle appears to behave much like heart in that in the early stages of hypoxia there is a transient elevation in ROS, whereas in chronic exposure to very severe hypoxia ...
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Increased reactive oxygen species (ROS) production is crucial to the remodelling that occurs in skeletal muscle in response to both exercise training and prolonged periods of disuse. This review discusses the redox-sensitive signalling pathways that are responsible for this ROS-induced skeletal muscle adaptation. We begin with a discussion of the sites of ROS production in skeletal muscle fibre...
متن کاملLow PO2 conditions induce reactive oxygen species formation during contractions in single skeletal muscle fibers
Li Zuo, Amy Shiah, William J. Roberts, Michael T. Chien, Peter D. Wagner, and Michael C. Hogan Department of Medicine, University of California, San Diego, La Jolla, California; Department of Biological Sciences, Oakland University, Rochester, Michigan; Respiratory Therapy Division, School of Health and Rehabilitation Sciences, Davis Heart and Lung Research Institute, The Ohio State University ...
متن کاملLow Po₂ conditions induce reactive oxygen species formation during contractions in single skeletal muscle fibers.
Contractions in whole skeletal muscle during hypoxia are known to generate reactive oxygen species (ROS); however, identification of real-time ROS formation within isolated single skeletal muscle fibers has been challenging. Consequently, there is no convincing evidence showing increased ROS production in intact contracting fibers under low Po₂ conditions. Therefore, we hypothesized that intrac...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2006
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.20.5.a1456-a